LOCUS       40924_34634        4284 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPigBase1.4 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Kojin Y, Mon H, Sakashita K, Tatsuke T, Miyagawa Y, Takahashi M, Lee
            J, Kawaguchi Y, Koga K, Kusakabe T.
  TITLE     Efficient RNA interference induction by long hairpin dsRNAs 
            expressed from chromosomes of the silkworm, Bombyx mori
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Kojin Y, Kusakabe T, Lee J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2006) Contact:Takahiro Kusakabe Kyushu University 
            Graduate School, Bioresource and Bioenviroonmental Sciences; 
            Laboratory of Silkworm Sicences, Hakozaki 6-10-1, Higashi-ku, 
            Fukuoka, Fukuoka 812-8581, Japan
REFERENCE   3  (bases 1 to 4284)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4284)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-MAR-2006) Contact:Takahiro Kusakabe Kyushu University Graduate 
            School, Bioresource and Bioenviroonmental Sciences; Laboratory of 
            Silkworm Sicences, Hakozaki 6-10-1, Higashi-ku, Fukuoka, Fukuoka 
            812-8581, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..430
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      551..1139
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(1442..1666)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1736..2266)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(2455..2483)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   2868..2880
                     /note="piggyBac transposable element, TR R; terminal repeat
                     of piggyBac"
     repeat_region   2884..2902
                     /label=piggyBac transposable element, IR R
                     /note="piggyBac transposable element, IR R"
     protein_bind    complement(3195..3228)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3239..3248
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3307..3401
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     repeat_region   3629..3648
                     /label=piggyBac transposable element, IR L
                     /note="piggyBac transposable element, IR L"
     repeat_region   3679..3691
                     /note="piggyBac transposable element, TR L; terminal repeat
                     of piggyBac"
     mobile_element  complement(3937..4102)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat aaaaggatct aggtgaagat cctttttgat aatctcatga
      481 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      541 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      601 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      661 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
      721 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      781 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      841 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      901 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
      961 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1021 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1081 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1141 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1201 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1261 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1321 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcagaccag
     1381 ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa tggatgccct gcgtaagcgg
     1441 gtgtgggcgg acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag
     1501 tcttaaacta gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca
     1561 tactggactt ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt
     1621 cgtattaaag aggggcgtgg ccaagggcat ggtaaagact atattcgcgg cgttgtgaca
     1681 atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg
     1741 cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga
     1801 gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg
     1861 cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct
     1921 cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca aacctgggca
     1981 gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga
     2041 atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg
     2101 tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt
     2161 ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt
     2221 tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat
     2281 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
     2341 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     2401 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
     2461 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
     2521 cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag
     2581 cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc
     2641 aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg
     2701 cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc
     2761 gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct acgacggtat
     2821 cgataagctt gaaagtttag gtcgagtaaa gcgcaaatct tttttaaccc tagaaagata
     2881 gtctgcgtaa aattgacgca tgcattcttg aaatattgct ctctctttct aaatagcgcg
     2941 aatccgtcgc tgtgcattta ggacatctca gtcgccgctt ggagctcccg tgaggcgtgc
     3001 ttgtcaatgc ggtaagtgtc actgattttg aactataacg accgcgtgag tcaaaatgac
     3061 gcatgattat cttttacgtg acttttaaga tttaactcat acgataatta tattgttatt
     3121 tcatgttcta cttacgtgat aacttattat atatatattt tcttgttata gatatccatc
     3181 acactggcgg ccgcataact tcgtatagca tacattatac gaagttatca gtcgacggcc
     3241 atggtggcca tttaaatatt accctgttat ccctagcgga tccgagtacg cagcatgcct
     3301 gcaggtcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
     3361 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagcgc
     3421 cggagtataa atctagtcga gcatgcatct agagaaaagt tttgttactt tatagaagaa
     3481 attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat
     3541 ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat
     3601 aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa
     3661 cgtacgtcac aatatgatta tctttctagg gttaaataat agtttctaat ttttttatta
     3721 ttcagcctgc tgtcgtgaat accgtatatc tcaacgctgt ctgtgagatt gtcgtattct
     3781 agccttttta gtttttcgct catcgacttg atattgtccg acacattttc gtcgatttgc
     3841 gttttgatca aagacttgag cagagacacg ttaatcaact gttcaaattg atccatatta
     3901 acgatggggg atcactgctt gagcctagga gatccgaacc agataagtga aatctagttc
     3961 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat
     4021 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc
     4081 ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac
     4141 atcctgccaa ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat
     4201 gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt
     4261 atttgcagcc tgaatggcga atgg
//
